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A Lateral Flow Biosensor for the Detection of Single Nucleotide Polymorphisms.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Zeng Lingwen, Xiao Zhuo

Abstract excerpt

A lateral flow biosensor (LFB) is introduced for the detection of single nucleotide polymorphisms (SNPs). The assay is composed of two steps: circular strand displacement reaction and lateral flow biosensor detection. In step 1, the nucleotide at SNP site is recognized by T4 DNA ligase and the signal is amplified by strand displacement DNA polymerase, which can be accomplished at a constant temperature. In step...

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Biosensing Techniques
Genotype
Gold
Immunoconjugates
Metal Nanoparticles
Nucleic Acid Amplification Techniques

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Polymorphism, Single Nucleotide

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PMID: 28299703
DOI: 10.1007/978-1-4939-6911-1_27

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