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Article

Flexible and rapid construction of viral chimeras applied to hepatitis C virus.

The Journal of general virology - 1 Sept 2016

McClure C Patrick, Urbanowicz Richard A, King Barnabas J, Cano-Crespo Sara, Tarr Alexander W, Ball Jonathan K

Abstract excerpt

A novel and broadly applicable strategy combining site-directed mutagenesis and DNA assembly for constructing seamless viral chimeras is described using hepatitis C virus (HCV) as an exemplar. Full-length HCV genomic cloning cassettes, which contained flexibly situated restriction endonuclease sites, were prepared via a single, site-directed mutagenesis reaction and digested to receive PCR-amplified virus...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 27329374
DOI: 10.1099/jgv.0.000530
PMCID: PMC5042125

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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