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Article

Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases: Insights into Donor and Acceptor Subsites.

Biochemistry - 24 Jul 2018

Madhuprakash Jogi, Dalhus Bjørn, Rani T Swaroopa, Podile Appa Rao, Eijsink Vincent G H, Sørlie Morten

Abstract excerpt

Understanding features that determine transglycosylation (TG) activity in glycoside hydrolases is important because it would allow the construction of enzymes that can catalyze controlled synthesis of oligosaccharides. To increase TG activity in two family 18 chitinases, chitinase D from Serratia proteamaculans ( SpChiD) and chitinase A from Serratia marcescens ( SmChiA), we have mutated residues important for...

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Identifiers

PMID: 29939724
DOI: 10.1021/acs.biochem.8b00381

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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