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Article

A simple method for isolation and construction of markerless cyanobacterial mutants defective in acyl-acyl carrier protein synthetase.

Applied microbiology and biotechnology - 1 Dec 2016

Kojima Kouji, Keta Sumie, Uesaka Kazuma, Kato Akihiro, Takatani Nobuyuki, Ihara Kunio, Omata Tatsuo, Aichi Makiko

Abstract excerpt

Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) secrete free fatty acids (FFAs) into the external medium and hence have been used for the studies aimed at photosynthetic production of biofuels. While the wild-type strain of Synechocystis sp. PCC 6803 is highly sensitive to exogenously added linolenic acid, mutants defective in the aas gene are known to be resistant to the externally...

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Identifiers

PMID: 27704180
DOI: 10.1007/s00253-016-7850-8
PMCID: PMC5102962

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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