Article
A Combination of CRISPR/Cas9 and Standardized RNAi as a Versatile Platform for the Characterization of Gene Function.
G3 (Bethesda, Md.) - 9 Aug 2016
Wissel Sebastian, Kieser Anja, Yasugi Tetsuo, Duchek Peter, Roitinger Elisabeth, Gokcezade Joseph, Steinmann Victoria, Gaul Ulrike, Mechtler Karl, Förstemann Klaus, Knoblich Jürgen A, Neumüller Ralph A
Abstract excerpt
Traditional loss-of-function studies in Drosophila suffer from a number of shortcomings, including off-target effects in the case of RNA interference (RNAi) or the stochastic nature of mosaic clonal analysis. Here, we describe minimal in vivo GFP interference (miGFPi) as a versatile strategy to characterize gene function and to conduct highly stringent, cell type-specific loss-of-function experiments in...
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