Article
Extending the linker region increases the activity of the Bacillus subtilis cellulase CelI15.
Biotechnology letters - 1 Sept 2016
Li Pingping, Zhou Yang, Li Qian, Zhang Chong, Sun Zhaowei, Tian Liuyang, Weng Haibo
Abstract excerpt
OBJECTIVES: To investigate the effect of the linker region (LR) on the enzymatic activity, stability, and flexibility of Bacillus subtilis cellulase CelI15, six mutants were constructed that contained increasing numbers of the LR. RESULTS: The CelI15 mutant with three copies of the LR (approx. 57 amino acids) showed the highest activity, which was almost 20 % greater than that of wild type CelI15. The stability...
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