Article
Iterative combinatorial mutagenesis as an effective strategy for generation of deacetoxycephalosporin C synthase with improved activity toward penicillin G.
Applied and environmental microbiology - 1 Nov 2012
Ji Junjie, Fan Keqiang, Tian Xiuyun, Zhang Xia, Zhang Yuxiu, Yang Keqian
Abstract excerpt
An iterative combinatorial mutagenesis (ICM) strategy was used to engineer deacetoxycephalosporin C synthase of Streptomyces clavuligerus (scDAOCS) for improved activity toward penicillin G. Seven mutational sites were repeatedly combined onto a starter mutant (C155Y Y184H V275I C281Y) of scDAOCS. Eleven improved combinatorial mutants were identified from 24 mutants in four rounds of ICM.
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.