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Article

Active site mutations of recombinant deacetoxycephalosporin C synthase.

Biochemical and biophysical research communications - 22 Mar 2002

Lee Hwei-Jen, Schofield Christopher J, Lloyd Matthew D

Abstract excerpt

Site-directed mutagenesis of active site residues of deacetoxycephalosporin C synthase active site residues was carried out to investigate their role in catalysis. The following mutations were made and their effects on the conversion of 2-oxoglutarate and the oxidation of penicillin N or G were assessed: M180F, G299N, G300N, Y302S, Y302F/G300A, Y302E, Y302H, and N304A. The Y302S, Y302E, and Y302H mutations...

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Identifiers

PMID: 11890672
DOI: 10.1006/bbrc.2002.6620

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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