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Article

Rapid DNA extraction protocol for detection of alpha-1 antitrypsin deficiency from dried blood spots by real-time PCR.

Advances in experimental medicine and biology - 1 Jan 2013

Struniawski R, Szpechcinski A, Poplawska B, Skronski M, Chorostowska-Wynimko J

Abstract excerpt

The dried blood spot (DBS) specimens have been successfully employed for the large-scale diagnostics of α1-antitrypsin (AAT) deficiency as an easy to collect and transport alternative to plasma/serum. In the present study we propose a fast, efficient, and cost effective protocol of DNA extraction from dried blood spot (DBS) samples that provides sufficient quantity and quality of DNA and effectively eliminates...

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PMID: 22836616
DOI: 10.1007/978-94-007-4549-0_5

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