Article
[DNA extraction from coagulated human blood for application in genotyping techniques for human leukocyte antigen and immunoglobulin-like receptors].
Revista da Sociedade Brasileira de Medicina Tropical - 1 Jan 2000
Cardozo Daniela Maira, Guelsin Gláucia Andréia, Clementino Samaia Laface, Melo Fabiano Cavalcante de, Braga Marco Antônio, Souza Cleonice de, Moliterno Ricardo Alberto, Visentainer Jeane Eliete Laguila
Abstract excerpt
The objective of this study was to standardize a method for extracting high-quality DNA from samples of coagulated blood. Forty-eight samples of human coagulated blood were used for DNA extraction by means of the EZ-DNA commercial kit (Biological Industries, Beit Haemek, Israel), the Neoscience column kit (One Lambda Inc., San Diego, CA, USA) and a modified salting-out method. Only the salting-out method was able...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.