Article
Cas9 Functionally Opens Chromatin.
PloS one - 1 Jan 2016
Barkal Amira A, Srinivasan Sharanya, Hashimoto Tatsunori, Gifford David K, Sherwood Richard I
Abstract excerpt
Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to...
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