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Article

A Novel Pretreatment-Free Duplex Chamber Digital PCR Detection System for the Absolute Quantitation of GMO Samples.

International journal of molecular sciences - 18 Mar 2016

Zhu Pengyu, Wang Chenguang, Huang Kunlun, Luo Yunbo, Xu Wentao

Abstract excerpt

Digital polymerase chain reaction (PCR) has developed rapidly since it was first reported in the 1990s. However, pretreatments are often required during preparation for digital PCR, which can increase operation error. The single-plex amplification of both the target and reference genes may cause uncertainties due to the different reaction volumes and the matrix effect. In the current study, a quantitative...

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Identifiers

PMID: 26999129
DOI: 10.3390/ijms17030402
PMCID: PMC4813257

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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