Article
A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids.
BMC molecular biology - 4 Jun 2008
Yang Litao, Liang Wanqi, Jiang Lingxi, Li Wenquan, Cao Wei, Wilson Zoe A, Zhang Dabing
Abstract excerpt
BACKGROUND: Real-time PCR techniques are being widely used for nucleic acids analysis, but one limitation of current frequently employed real-time PCR is the high cost of the labeled probe for each target molecule. RESULTS: We describe a real-time PCR technique employing attached universal duplex probes (AUDP), which has the advantage of generating fluorescence by probe hydrolysis and strand displacement over...
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