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Article

Obtaining a mutant of Bacillus amyloliquefaciens xylanase A with improved catalytic activity by directed evolution.

Enzyme and microbial technology - 1 May 2016

Xu Xin, Liu Ming-qi, Huo Wen-kang, Dai Xian-jun

Abstract excerpt

This study aimed to obtain xylanase exhibiting improved enzyme properties to satisfy the requirements for industrial applications. The baxA gene encoding Bacillus amyloliquefaciens xylanase A was mutated by error-prone touchdown PCR. The mutant, pCbaxA50, was screened from the mutant library by using the 96-well plate high-throughput screening method. Sequence alignment revealed the identical mutation point S138T...

Topics

Bacillus amyloliquefaciens
Bacterial Proteins
Directed Molecular Evolution
Endo-1,4-beta Xylanases
Enzyme Stability
Genes, Bacterial
Hydrolysis
Kinetics
Models, Molecular
Mutation
Protein Conformation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Recombinant Proteins

Structural Homology, Protein

Substrate Specificity

Xylans

Source

Identifiers

PMID: 26992794
DOI: 10.1016/j.enzmictec.2016.02.001

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