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Article

Site-directed mutagenesis of GH10 xylanase A from Penicillium canescens for determining factors affecting the enzyme thermostability.

International journal of biological macromolecules - 1 Nov 2017

Denisenko Yury A, Gusakov Alexander V, Rozhkova Aleksandra M, Osipov Dmitry O, Zorov Ivan N, Matys Veronika Yu, Uporov Igor V, Sinitsyn Arkady P

Abstract excerpt

In order to investigate factors affecting the thermostability of GH10 xylanase A from Penicillium canescens (PcXylA) and to obtain its more stable variant, the wild-type (wt) enzyme and its mutant forms, carrying single amino acid substitutions, were cloned and expressed in Penicillium verruculosum B1-537 (niaD-) auxotrophic strain under the control of the cbh1 gene promoter. The recombinant PcXylA-wt and I6V,...

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Identifiers

PMID: 28634062
DOI: 10.1016/j.ijbiomac.2017.06.079

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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