Article
T cell-specific inactivation of mouse CD2 by CRISPR/Cas9.
Scientific reports - 23 Feb 2016
Beil-Wagner Jane, Dössinger Georg, Schober Kilian, vom Berg Johannes, Tresch Achim, Grandl Martina, Palle Pushpalatha, Mair Florian, Gerhard Markus, Becher Burkhard, Busch Dirk H, Buch Thorsten
Abstract excerpt
The CRISPR/Cas9 system can be used to mutate target sequences by introduction of double-strand breaks followed by imprecise repair. To test its use for conditional gene editing we generated mice transgenic for CD4 promoter-driven Cas9 combined with guide RNA targeting CD2. We found that within CD4(+) and CD8(+) lymphocytes from lymph nodes and spleen 1% and 0.6% were not expressing CD2, respectively. T cells...
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