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Article

Efficient CRISPR/Cas9 Gene Editing in Uncultured Naive Mouse T Cells for In Vivo Studies.

Journal of immunology (Baltimore, Md. : 1950) - 15 Apr 2020

Nüssing Simone, House Imran G, Kearney Conor J, Chen Amanda X Y, Vervoort Stephin J, Beavis Paul A, Oliaro Jane, Johnstone Ricky W, Trapani Joseph A, Parish Ian A

Abstract excerpt

CRISPR/Cas9 technologies have revolutionized our understanding of gene function in complex biological settings, including T cell immunology. Current CRISPR-mediated gene editing strategies in T cells require in vitro stimulation or culture that can both preclude the study of unmanipulated naive T cells and alter subsequent differentiation. In this study, we demonstrate highly efficient gene editing within...

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Identifiers

PMID: 32152070
DOI: 10.4049/jimmunol.1901396

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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