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Article

Strand-biased cytosine deamination at the replication fork causes cytosine to thymine mutations in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America - 23 Feb 2016

Bhagwat Ashok S, Hao Weilong, Townes Jesse P, Lee Heewook, Tang Haixu, Foster Patricia L

Abstract excerpt

The rate of cytosine deamination is much higher in single-stranded DNA (ssDNA) than in double-stranded DNA, and copying the resulting uracils causes C to T mutations. To study this phenomenon, the catalytic domain of APOBEC3G (A3G-CTD), an ssDNA-specific cytosine deaminase, was expressed in an Escherichia coli strain defective in uracil repair (ung mutant), and the mutations that accumulated over thousands of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26839411
DOI: 10.1073/pnas.1522325113
PMCID: PMC4776466

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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