Article
Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions.
Nucleic acids research - 1 Dec 2010
Claesson Marcus J, Wang Qiong, O'Sullivan Orla, Greene-Diniz Rachel, Cole James R, Ross R Paul, O'Toole Paul W
Abstract excerpt
High-throughput molecular technologies can profile microbial communities at high resolution even in complex environments like the intestinal microbiota. Recent improvements in next-generation sequencing technologies allow for even finer resolution. We compared phylogenetic profiling of both longer (454 Titanium) sequence reads with shorter, but more numerous, paired-end reads (Illumina). For both approaches, we...
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