Article
A Genome-wide RNAi Screen for Microtubule Bundle Formation and Lysosome Motility Regulation in Drosophila S2 Cells.
Cell reports - 26 Jan 2016
Jolly Amber L, Luan Chi-Hao, Dusel Brendon E, Dunne Sara F, Winding Michael, Dixit Vishrut J, Robins Chloe, Saluk Jennifer L, Logan David J, Carpenter Anne E, Sharma Manu, Dean Deborah, Cohen Andrew R, Gelfand Vladimir I
Abstract excerpt
Long-distance intracellular transport of organelles, mRNA, and proteins ("cargo") occurs along the microtubule cytoskeleton by the action of kinesin and dynein motor proteins, but the vast network of factors involved in regulating intracellular cargo transport are still unknown. We capitalize on the Drosophila melanogaster S2 model cell system to monitor lysosome transport along microtubule bundles, which require...
Topics
- Animals
- Bayes Theorem
- Cells, Cultured
- Drosophila
- Drosophila Proteins
- Dyneins
- Genome
- Lysosomes
- Microtubules
- Phenotype
- RNA Interference
- RNA, Double-Stranded
- Time-Lapse Imaging