Article
Validation of targeted next-generation sequencing for RAS mutation detection in FFPE colorectal cancer tissues: comparison with Sanger sequencing and ARMS-Scorpion real-time PCR.
BMJ open - 8 Jan 2016
Gao Jie, Wu Huanwen, Wang Li, Zhang Hui, Duan Huanli, Lu Junliang, Liang Zhiyong
Abstract excerpt
OBJECTIVE: To validate the targeted next-generation sequencing (NGS) platform-Ion Torrent PGM for KRAS exon 2 and expanded RAS mutations detection in formalin-fixed paraffin-embedded (FFPE) colorectal cancer (CRC) specimens, with comparison of Sanger sequencing and ARMS-Scorpion real-time PCR. SETTING: Beijing, China. PARTICIPANTS: 51 archived FFPE CRC samples (36 men, 15 women) were retrospectively randomly...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.