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Article

Enhanced GAD65 production in plants using the MagnICON transient expression system: Optimization of upstream production and downstream processing.

Biotechnology journal - 1 Mar 2016

Merlin Matilde, Gecchele Elisa, Arcalis Elsa, Remelli Sabrina, Brozzetti Annalisa, Pezzotti Mario, Avesani Linda

Abstract excerpt

Plants have emerged as competitive production platforms for pharmaceutical proteins that are required in large quantities. One example is the 65-kDa isoform of human glutamic acid decarboxylase (GAD65), a major autoimmune diabetes autoantigen that has been developed as a vaccine candidate for the primary prevention of diabetes. The expression of GAD65 in plants has been optimized but large-scale purification is...

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PMID: 26710327
DOI: 10.1002/biot.201500187

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