Article
Highly efficient heritable plant genome engineering using Cas9 orthologues from Streptococcus thermophilus and Staphylococcus aureus.
The Plant journal : for cell and molecular biology - 1 Dec 2015
Steinert Jeannette, Schiml Simon, Fauser Friedrich, Puchta Holger
Abstract excerpt
The application of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system of Streptococcus pyogenes (SpCas9) is currently revolutionizing genome engineering in plants. However, synthetic plant biology will require more complex manipulations of genomes and transcriptomes. The simultaneous addressing of different specific genomic sites with independent enzyme activities within the same...
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