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Article

Directed evolution of human scFvs in DT40 cells.

Protein engineering, design & selection : PEDS - 1 Feb 2016

Lim Alfred W Y, Williams Gareth T, Rada Cristina, Sale Julian E

Abstract excerpt

Cells that constitutively diversify their immunoglobulin genes can be used for selection of novel antibodies and for refining existing affinities and specificities. Here, we report an adaptation of the chicken DT40 system wherein its capacity for somatic hypermutation is harnessed to evolve human antibodies expressed as single-chain variable fragments (scFvs). Expression of membrane-anchored scFvs from within the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 26519451
DOI: 10.1093/protein/gzv058
PMCID: PMC4840456

Genes

CWC15

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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