Article
A Combination of Structural and Empirical Analyses Delineates the Key Contacts Mediating Stability and Affinity Increases in an Optimized Biotherapeutic Single-chain Fv (scFv).
The Journal of biological chemistry - 15 Jan 2016
Tu Chao, Terraube Virginie, Tam Amy Sze Pui, Stochaj Wayne, Fennell Brian J, Lin Laura, Stahl Mark, LaVallie Edward R, Somers Will, Finlay William J J, Mosyak Lydia, Bard Joel, Cunningham Orla
Abstract excerpt
Fully-human single-chain Fv (scFv) proteins are key potential building blocks of bispecific therapeutic antibodies, but they often suffer from manufacturability and clinical development limitations such as instability and aggregation. The causes of these scFv instability problems, in proteins that should be theoretically stable, remains poorly understood. To inform the future development of such molecules, we...
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