Article
In Vitro Mutational and Bioinformatics Analysis of the M71 Odorant Receptor and Its Superfamily.
PloS one - 1 Jan 2015
Bubnell Jaclyn, Jamet Sophie, Tomoiaga Delia, D'Hulst Charlotte, Krampis Konstantinos, Feinstein Paul
Abstract excerpt
We performed an extensive mutational analysis of the canonical mouse odorant receptor (OR) M71 to determine the properties of ORs that inhibit plasma membrane trafficking in heterologous expression systems. We employed the use of the M71::GFP fusion protein to directly assess plasma membrane localization and functionality of M71 in heterologous cells in vitro or in olfactory sensory neurons (OSNs) in vivo. OSN...
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