Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Multiple Hotspot Mutations Scanning by Single Droplet Digital PCR.

Clinical chemistry - 1 Feb 2018

Decraene Charles, Silveira Amanda B, Bidard François-Clément, Vallée Audrey, Michel Marc, Melaabi Samia, Vincent-Salomon Anne, Saliou Adrien, Houy Alexandre, Milder Maud, Lantz Olivier, Ychou Marc, Denis Marc G, Pierga Jean-Yves, Stern Marc-Henri, Proudhon Charlotte

Abstract excerpt

BACKGROUND: Progress in the liquid biopsy field, combined with the development of droplet digital PCR (ddPCR), has enabled noninvasive monitoring of mutations with high detection accuracy. However, current assays detect a restricted number of mutations per reaction. ddPCR is a recognized method for detecting alterations previously characterized in tumor tissues, but its use as a discovery tool when the mutation...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biopsy
Share to Circulating Tumor DNA

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to ErbB Receptors

Share to Genes, ras

Share to Limit of Detection

Share to Liquid Biopsy

Share to Molecular Probes

Share to Neoplasms

Source

Identifiers

PMID: 29122835
DOI: 10.1373/clinchem.2017.272518

Genes

EGFR
KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Multiple Hotspot Mutations Scanning by Single Droplet Digital PCR.PMID 29122835Read ↗

A Novel Multiplex Droplet Digital PCR Assay to Identify and Quantify KRAS Mutations in Clinical SpecimensSemantic proximity Read ↗

Multiplex Detection of Rare Mutations by Picoliter Droplet Based Digital PCR: Sensitivity and Specificity Considerations.Semantic proximity Read ↗

Atypical Droplet Digital Polymerase Chain Reaction Patterns That Indicate Uncommon but Clinically Actionable EGFR Mutations in Lung Cancer.Semantic proximity Read ↗

Efficient Genotyping of KRAS Mutant Non-Small Cell Lung Cancer Using a Multiplexed Droplet Digital PCR Approach.Semantic proximity Read ↗

Liquid Biopsy Hotspot Variant Assays: Analytical Validation for Application in Residual Disease Detection and Treatment Monitoring.Semantic proximity Read ↗

Monitoring KRAS mutations in circulating DNA and tumor cells using digital droplet PCR during treatment of KRAS-mutated lung adenocarcinoma.Semantic proximity Read ↗

Enhanced ratio of signals enables digital mutation scanning for rare allele detection.Semantic proximity Read ↗

Multiplex Enrichment and Detection of Rare KRAS Mutations in Liquid Biopsy Samples using Digital Droplet Pre-AmplificationSemantic proximity Read ↗

Multiplex Enrichment and Detection of Rare KRAS Mutations in Liquid Biopsy Samples using Digital Droplet Pre-AmplificationSemantic proximity Read ↗

Quantitative and sensitive detection of rare mutations using droplet-based microfluidics.Semantic proximity Read ↗

Non-invasive profiling of KRAS mutations in lung cancer using droplet digital polymerase chain reaction.Semantic proximity Read ↗

Development and Clinical Validation of Discriminatory Multitarget Digital Droplet PCR Assays for the Detection of Hot Spot KRAS and NRAS Mutations in Cell-Free DNA.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.