Article
Troponin I Mutations R146G and R21C Alter Cardiac Troponin Function, Contractile Properties, and Modulation by Protein Kinase A (PKA)-mediated Phosphorylation.
The Journal of biological chemistry - 13 Nov 2015
Cheng Yuanhua, Rao Vijay, Tu An-Yue, Lindert Steffen, Wang Dan, Oxenford Lucas, McCulloch Andrew D, McCammon J Andrew, Regnier Michael
Abstract excerpt
Two hypertrophic cardiomyopathy-associated cardiac troponin I (cTnI) mutations, R146G and R21C, are located in different regions of cTnI, the inhibitory peptide and the cardiac-specific N terminus. We recently reported that these regions may interact when Ser-23/Ser-24 are phosphorylated, weakening the interaction of cTnI with cardiac TnC. Little is known about how these mutations influence the affinity of...
Topics
- Amino Acid Substitution
- Animals
- Arginine
- Calcium
- Cyclic AMP-Dependent Protein Kinases
- Cysteine
- Glycine
- Humans
- Male
- Molecular Dynamics Simulation
- Mutation
- Myocardial Contraction
- Myofibrils