Article
Cas9 gRNA engineering for genome editing, activation and repression.
Nature methods - 1 Nov 2015
Kiani Samira, Chavez Alejandro, Tuttle Marcelle, Hall Richard N, Chari Raj, Ter-Ovanesyan Dmitry, Qian Jason, Pruitt Benjamin W, Beal Jacob, Vora Suhani, Buchthal Joanna, Kowal Emma J K, Ebrahimkhani Mohammad R, Collins James J, Weiss Ron, Church George
Abstract excerpt
We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
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