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Article

Balancing between affinity and speed in target DNA search by zinc-finger proteins via modulation of dynamic conformational ensemble.

Proceedings of the National Academy of Sciences of the United States of America - 15 Sept 2015

Zandarashvili Levani, Esadze Alexandre, Vuzman Dana, Kemme Catherine A, Levy Yaakov, Iwahara Junji

Abstract excerpt

Although engineering of transcription factors and DNA-modifying enzymes has drawn substantial attention for artificial gene regulation and genome editing, most efforts focus on affinity and specificity of the DNA-binding proteins, typically overlooking the kinetic properties of these proteins. However, a simplistic pursuit of high affinity can lead to kinetically deficient proteins that spend too much time at...

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Identifiers

PMID: 26324943
DOI: 10.1073/pnas.1507726112
PMCID: PMC4577168

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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