Article
Long- and Short-Range Electrostatic Fields in GFP Mutants: Implications for Spectral Tuning.
Scientific reports - 19 Aug 2015
Drobizhev M, Callis P R, Nifosì R, Wicks G, Stoltzfus C, Barnett L, Hughes T E, Sullivan P, Rebane A
Abstract excerpt
The majority of protein functions are governed by their internal local electrostatics. Quantitative information about these interactions can shed light on how proteins work and allow for improving/altering their performance. Green fluorescent protein (GFP) and its mutation variants provide unique optical windows for interrogation of internal electric fields, thanks to the intrinsic fluorophore group formed inside...
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