Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

DNA sequencing with MspA: Molecular Dynamics simulations reveal free-energy differences between sequencing and non-sequencing mutants.

Scientific reports - 10 Aug 2015

Manara Richard M A, Wallace E Jayne, Khalid Syma

Abstract excerpt

MspA has been identified as a promising candidate protein as a component of a nanopore-based DNA-sequencing device. However the wildtype protein must be engineered to incorporate all of the features desirable for an accurate and efficient device. In the present study we have utilized atomistic molecular dynamics to perform umbrella-sampling calculations to calculate the potential of mean force (PMF) profiles for...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA
Share to Hydrogen Bonding
Share to Molecular Dynamics Simulation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutation

Share to Porins

Share to Protein Structure, Tertiary

Share to Sequence Analysis, DNA

Share to Thermodynamics

Source

Open in PubMed Open full text

Identifiers

PMID: 26255609
DOI: 10.1038/srep12783
PMCID: PMC4530457

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

DNA sequencing with MspA: Molecular Dynamics simulations reveal free-energy differences between sequencing and non-sequencing mutants.PMID 26255609Read ↗

Molecular dynamics study of MspA arginine mutants predicts slow DNA translocations and ion current blockades indicative of DNA sequence.Semantic proximity Read ↗

Control of subunit stoichiometry in single-chain MspA nanoporesSemantic proximity Read ↗

Effects of Nanopore Charge Decorations on the Translocation Dynamics of DNA.Semantic proximity Read ↗

Nucleotide discrimination with DNA immobilized in the MspA nanopore.Semantic proximity Read ↗

Sequencing of <i>α</i> -synuclein Intrinsically Disordered Protein in MoS <sub>2</sub> NanoporesSemantic proximity Read ↗

Molecular dynamics simulations of DNA within a nanopore: arginine-phosphate tethering and a binding/sliding mechanism for translocation.Semantic proximity Read ↗

The mutate-and-map protocol for inferring base pairs in structured RNA.Semantic proximity Read ↗

Molecular dynamics simulations reveal that AEDANS is an inert fluorescent probe for the study of membrane proteins.Semantic proximity Read ↗

Threading single proteins through pores to compare their energy landscapes.Semantic proximity Read ↗

Free-energy landscapes of membrane co-translocational protein unfolding.Semantic proximity Read ↗

The human mitochondrial single-stranded DNA-binding protein displays distinct kinetics and thermodynamics of DNA binding and exchange.Semantic proximity Read ↗

Altering the speed of a DNA packaging motor from bacteriophage T4.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.