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Rapid detection of α-thalassaemia alleles of --(SEA)/, -α(3.7)/ and -α(4.2)/ using a dual labelling, self-quenching hybridization probe/melting curve analysis.

Molecular and cellular probes - 1 Dec 2015

Gao Lan, Liu Yanhui, Sun Manna, Zhao Ying, Xie Rungui, He Yi, Xu Wanfang, Liu Jianxin, Lin Yangyang, Lou Jiwu

Abstract excerpt

OBJECTIVES: The aim of the study was to set up an alternative automatic molecular diagnostic method for deletional α-thalassaemia mutations without gel electrophoresis. METHODS: Based on the sequence variation within the two Z boxes and melting curve analysis of dually labelled probes, a real-time PCR assay was developed and validated for the rapid detection of major α-genotypes (--(SEA)/αα, --(SEA)/-α(3.7),...

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PMID: 26190814
DOI: 10.1016/j.mcp.2015.07.004

Genes

SEA

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