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Article

A Nested Asymmetric PCR Melting Curve Assay for One-Step Genotyping of Nondeletional α-Thalassemia Mutations.

The Journal of molecular diagnostics : JMD - 1 Jun 2020

Qin Jiachun, Xu Mingli, Zhang Qiang, Wen Xiaojun, He Sheng, Zhou Yong, Liu Haiping, Zhou Wanjun

Abstract excerpt

A rapid DNA-based assay is essential for clinical diagnosis and mass screening in thalassemia-prevention programs. Because of high homology and guanine-cytosine-rich and complex second structure of α-globin genes, it is rather difficult to develop a feasible and simple method for α-thalassemia genotyping. In this study, a strategy of nested asymmetric PCR melting curve analysis was designed to tackle these...

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Identifiers

PMID: 32482310
DOI: 10.1016/j.jmoldx.2020.03.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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