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Article

Temperature Effects on Kinetic Parameters and Substrate Affinity of Cel7A Cellobiohydrolases.

The Journal of biological chemistry - 4 Sept 2015

Sørensen Trine Holst, Cruys-Bagger Nicolaj, Windahl Michael Skovbo, Badino Silke Flindt, Borch Kim, Westh Peter

Abstract excerpt

We measured hydrolytic rates of four purified cellulases in small increments of temperature (10-50 °C) and substrate loads (0-100 g/liter) and analyzed the data by a steady state kinetic model that accounts for the processive mechanism. We used wild type cellobiohydrolases (Cel7A) from mesophilic Hypocrea jecorina and thermophilic Rasamsonia emersonii and two variants of these enzymes designed to elucidate the...

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Identifiers

PMID: 26183777
DOI: 10.1074/jbc.M115.658930
PMCID: PMC4571970

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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