Article
Effects of pH and high ionic strength on the adsorption and activity of native and mutated cellobiohydrolase I from Trichoderma reesei.
Proteins - 1 Aug 1995
Reinikainen T, Teleman O, Teeri T T
Abstract excerpt
Cellobiohydrolase I (CBHI) is the major cellulase of Trichoderma reesei. The enzyme contains a discrete cellulose-binding domain (CBD), which increases its binding and activity on crystalline cellulose. We studied cellulase-cellulose interactions using site-directed mutagenesis on the basis of the three-dimensional structure of the CBD of CBHI. Three mutant proteins which have earlier been produced in...
Topics
- Adsorption
- Binding Sites
- Cellulose
- Cellulose 1,4-beta-Cellobiosidase
- Glycoside Hydrolases
- Glycosylation
- Hydrogen-Ion Concentration
- Magnesium Sulfate
- Models, Molecular
- Mutation
- Osmolar Concentration