Article
Costs and benefits of processivity in enzymatic degradation of recalcitrant polysaccharides.
Proceedings of the National Academy of Sciences of the United States of America - 28 Nov 2006
Horn Svein J, Sikorski Pawel, Cederkvist Jannicke B, Vaaje-Kolstad Gustav, Sørlie Morten, Synstad Bjørnar, Vriend Gert, Vårum Kjell M, Eijsink Vincent G H
Abstract excerpt
Many enzymes that hydrolyze insoluble crystalline polysaccharides such as cellulose and chitin guide detached single-polymer chains through long and deep active-site clefts, leading to processive (stepwise) degradation of the polysaccharide. We have studied the links between enzyme efficiency and processivity by analyzing the effects of mutating aromatic residues in the substrate-binding groove of a processive...
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