Article
Alleviation of off-target effects from vector-encoded shRNAs via codelivered RNA decoys.
Proceedings of the National Academy of Sciences of the United States of America - 28 Jul 2015
Mockenhaupt Stefan, Grosse Stefanie, Rupp Daniel, Bartenschlager Ralf, Grimm Dirk
Abstract excerpt
Exogenous RNAi triggers such as shRNAs ideally exert their activities exclusively via the antisense strand that binds and silences designated target mRNAs. However, in principle, the sense strand also possesses silencing capacity that may contribute to adverse RNAi side effects including off-target gene regulation. Here, we address this concern with a novel strategy that reduces sense strand activity of...
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