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Article

Versatile Viral Vector Strategies for Postscreening Target Validation and RNAi ON-Target Control.

Journal of biomolecular screening - 1 Sept 2015

Christel Carl J, Schmied Patricia, Jagusch Verena, Schrödel Silke, Thirion Christian, Schmitt Kathrin, Salomon Michael

Abstract excerpt

Our approach aims to optimize postscreening target validation strategies using viral vector-driven RNA interference (RNAi) cell models. The RNAiONE validation platform is an array of plasmid-based expression vectors that each drives tandem expression of the gene of interest (GOI) with one small hairpin RNA (shRNA) from a set of computed candidate sequences. The best-performing shRNA (>85% silencing efficiency) is...

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Identifiers

PMID: 25873558
DOI: 10.1177/1087057115581803

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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