Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell lines.

doi:10.1093/nar/gkt836

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Article

Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell lines.

Nucleic acids research - 1 Nov 2013

Börner Kathleen, Niopek Dominik, Cotugno Gabriella, Kaldenbach Michaela, Pankert Teresa, Willemsen Joschka, Zhang Xian, Schürmann Nina, Mockenhaupt Stefan, Serva Andrius, Hiet Marie-Sophie, Wiedtke Ellen, Castoldi Mirco, Starkuviene Vytaute, Erfle Holger, Gilbert Daniel F, Bartenschlager Ralf, Boutros Michael, Binder Marco, Streetz Konrad, Kräusslich Hans-Georg, Grimm Dirk

Abstract excerpt

As the only mammalian Argonaute protein capable of directly cleaving mRNAs in a small RNA-guided manner, Argonaute-2 (Ago2) is a keyplayer in RNA interference (RNAi) silencing via small interfering (si) or short hairpin (sh) RNAs. It is also a rate-limiting factor whose saturation by si/shRNAs limits RNAi efficiency and causes numerous adverse side effects. Here, we report a set of versatile tools and widely...

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Identifiers

PMID: 24049077
DOI: 10.1093/nar/gkt836
PMCID: PMC3834839

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