Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A Universal Positive-Negative Selection System for Gene Targeting in Plants Combining an Antibiotic Resistance Gene and Its Antisense RNA.

Plant physiology - 1 Sept 2015

Nishizawa-Yokoi Ayako, Nonaka Satoko, Osakabe Keishi, Saika Hiroaki, Toki Seiichi

Abstract excerpt

Gene targeting (GT) is a useful technology for accurate genome engineering in plants. A reproducible approach based on a positive-negative selection system using hygromycin resistance and the diphtheria toxin A subunit gene as positive and negative selection markers, respectively, is now available. However, to date, this selection system has been applied exclusively in rice (Oryza sativa). To establish a...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Blotting, Southern
Share to Chromosome Segregation

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Drug Resistance, Microbial

Share to Gene Knockout Techniques

Share to Gene Targeting

Share to Gentamicins

Share to RNA, Antisense

Source

Open in PubMed Open full text

Identifiers

PMID: 26143254
DOI: 10.1104/pp.15.00638
PMCID: PMC4577407

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A Universal Positive-Negative Selection System for Gene Targeting in Plants Combining an Antibiotic Resistance Gene and Its Antisense RNA.PMID 26143254Read ↗

Modification of endogenous natural genes by gene targeting in rice and other higher plants.Semantic proximity Read ↗

A mutated cytosine deaminase gene, codA (D314A), as an efficient negative selection marker for gene targeting in rice.Semantic proximity Read ↗

A selection strategy in plant transformation based on antisense oligodeoxynucleotide inhibition.Semantic proximity Read ↗

A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure.Semantic proximity Read ↗

Broad-Spectrum Blast Resistance: Harnessing a Natural Allele of a Transcription Factor in Rice.Semantic proximity Read ↗

Dual screening for targeted gene replacement mutant in Magnaporthe oryzae with GUS as negative marker.Semantic proximity Read ↗

Rapid selection of transgenic mammalian cells via diphtheria toxin resistanceSemantic proximity Read ↗

Removal of antibiotic resistance genes from transgenic tobacco plastids.Semantic proximity Read ↗

Plant genetic transformation efficiency of selected Malaysian rice based on selectable marker gene (hptII).Semantic proximity Read ↗

A dual selection based, targeted gene replacement tool for Magnaporthe grisea and Fusarium oxysporum.Semantic proximity Read ↗

Application of gene targeting to designed mutation breeding of high-tryptophan rice.Semantic proximity Read ↗

Gene trap mutagenesis-based forward genetic approach reveals that the tumor suppressor OVCA1 is a component of the biosynthetic pathway of diphthamide on elongation factor 2.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.