A mutated cytosine deaminase gene, codA (D314A), as an efficient negative selection marker for gene targeting in rice.

Abstract excerpt

Gene targeting (GT) is a powerful tool manipulating a gene of interest in a given genome specifically and precisely. To achieve efficient GT in higher plants, both positive and negative selection markers are required. In particular, a strong negative selection system is needed for enrichment of cells to eliminate those cells in which random integration of the introduced DNA has occurred in GT experiments....

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