Article
Plant genetic transformation efficiency of selected Malaysian rice based on selectable marker gene (hptII).
Pakistan journal of biological sciences : PJBS - 1 Apr 2014
Htwe Nwe Nwe, Ling Ho Chai, Zaman Faridah Qamaruz, Maziah Mahmood
Abstract excerpt
Rice is one of the most important cereal crops with great potential for biotechnology progress. In transformation method, antibiotic resistance genes are routinely used as powerful markers for selecting transformed cells from surrounding non-transformed cells. In this study, the toxicity level of hygromycin was optimized for two selected mutant rice lines, MR219 line 4 and line 9. The mature embryos were isolated...
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