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Article

Dual screening for targeted gene replacement mutant in Magnaporthe oryzae with GUS as negative marker.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Jan 2009

Wang Jiaoyu, Zhang Zhen, Du Xinfa, Chai Rongyao, Mao Xueqin, Qiu Haiping, Wang Yanli, Sun Guochang

Abstract excerpt

To improve the efficiency of targeted gene replacement (TGR), a dual screen (DS) system with gusA gene as negative selective marker (GUS-DS) was developed in Magnaporthe oryzae. First, we tested the endogenous beta-glucuronidase (GUS) activities of 78 fungal strains. All tested strains were GUS-, only with 3 exceptions. Whereas, after the gusA being introduced in, M. oryzae, Fusarium oxysporum and Colletotrichum...

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PMID: 19441238

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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