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Article

Cut-and-paste-based cloning strategy for large gene site-directed mutagenesis.

Genetics and molecular research : GMR - 25 May 2015

Wang C, Wang T Y, Zhang L Y, Gao X J, Wang X W, Jin C J

Abstract excerpt

Site-directed mutagenesis is an essential technique for investigating the mechanisms of gene regulation on a molecular level, as well as for exploring post-translational modifications and functional structure at the protein level. Polymerase chain reaction combining in vitro synthesis of oligonucleotide primers allows for site-directed mutation to be performed with ease. However, site-directed mutagenesis is...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26125756
DOI: 10.4238/2015.May.25.10

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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