Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome.

Nature methods - 1 Aug 2015

Linder Bastian, Grozhik Anya V, Olarerin-George Anthony O, Meydan Cem, Mason Christopher E, Jaffrey Samie R

Abstract excerpt

N(6)-methyladenosine (m6A) is the most abundant modified base in eukaryotic mRNA and has been linked to diverse effects on mRNA fate. Current mapping approaches localize m6A residues to transcript regions 100-200 nt long but cannot identify precise m6A positions on a transcriptome-wide level. Here we developed m6A individual-nucleotide-resolution cross-linking and immunoprecipitation (miCLIP) and used it to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Adenosine
Share to Antibodies
Share to Cross-Linking Reagents

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to HEK293 Cells

Share to Methylation

Share to Nucleotide Mapping

Share to RNA, Small Nucleolar

Share to Ultraviolet Rays

Source

Open in PubMed Open full text

Identifiers

PMID: 26121403
DOI: 10.1038/nmeth.3453
PMCID: PMC4487409

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome.PMID 26121403Read ↗

Single-base mapping of m <sup>6</sup> A by an antibody-independent methodSemantic proximity Read ↗

Sequence- and structure-specific cytosine-5 mRNA methylation by NSUN6Semantic proximity Read ↗

m6A Modification in Coding and Non-coding RNAs: Roles and Therapeutic Implications in Cancer.Semantic proximity Read ↗

i <sup>6</sup> A-seq maps <i>N</i> <sup>6</sup> -isopentenyladenosine and uncovers its role as a regulator of mRNA stability through recruitment of DIS3L2Semantic proximity Read ↗

Comprehensive analysis across mammalian tissues and cells decipher the underlying mechanism of m6A specificitySemantic proximity Read ↗

Long-read RNA sequencing reveals allele-specific N <sup>6</sup> -methyladenosine modificationsSemantic proximity Read ↗

Identification of m6A residues at single-nucleotide resolution using eCLIP and an accessible custom analysis pipeline.Semantic proximity Read ↗

Long-read RNA sequencing reveals allele-specific N 6-methyladenosine modifications.Semantic proximity Read ↗

Identification of differentially methylated single-nucleotide m <sup>6</sup> A sites by incorporating site-specific antibody specificitySemantic proximity Read ↗

Antibody-free enzyme-assisted chemical labeling for detection of transcriptome-wide <i>N</i> <sup>6</sup> -methyladenosineSemantic proximity Read ↗

Landscape and Regulation of m6A and m6Am Methylome across Human and Mouse Tissues.Semantic proximity Read ↗

RMBase v2.0: deciphering the map of RNA modifications from epitranscriptome sequencing data.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.