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Article

Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.

Chemical communications (Cambridge, England) - 14 Jul 2011

Miyauchi Yusuke, Kourist Robert, Uemura Daisuke, Miyamoto Kenji

Abstract excerpt

Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).

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Identifiers

PMID: 21559536
DOI: 10.1039/c1cc11953b

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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