Article
Direct comparison study between droplet digital PCR and a combination of allele-specific PCR, asymmetric rapid PCR and melting curve analysis for the detection of BRAF V600E mutation in plasma from melanoma patients.
Clinical chemistry and laboratory medicine - 25 Oct 2020
Tzanikou Eleni, Haselmann Verena, Markou Athina, Duda Angelika, Utikal Jochen, Neumaier Michael, Lianidou Evi S
Abstract excerpt
Background In metastatic melanoma, 40%-50% of patients harbor a BRAF V600E mutation and are thereby eligible to receive a combined BRAF/MEK inhibitor therapy. Compared to standard-of-care tissue-based genetic testing, analysis of circulating tumor DNA (ctDNA) from blood enables a comprehensive as...
Topics
- Adult
- Aged
- Aged, 80 and over
- Circulating Tumor DNA
- Cohort Studies
- Female
- Humans
- Liquid Biopsy
- Male
- Melanoma
- Middle Aged
- Mutation
- Polymerase Chain Reaction
- Proto-Oncogene Proteins B-raf