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Efficient Genome Engineering of Toxoplasma gondii Using CRISPR/Cas9

27 Jun 2014

Abstract excerpt

Toxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp., the causative agents of malaria. Despite many advances, manipulating the T. gondii genome remains labor intensive, and is often restricted to lab-adapted strains or lines carrying mutations that enable selection. Here, we use the RNA-guided Cas9 nuclease to efficiently generate knockouts without...

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PMID: 24971596
DOI: 10.1371/journal.pone.0100450

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