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Article

Application of CRISPR/Cas9-Mediated Genome Editing in Leishmania.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Zhang Wen-Wei, Lypaczewski Patrick, Matlashewski Greg

Abstract excerpt

CRISPR-Cas9 is an RNA guided endonuclease derived from the bacterium Streptococcus pyogenes. Due to its simplicity, versatility, and high efficiency, it has been widely used for genome editing in a variety of organisms including the protozoan parasite Leishmania, the causative agent of human leishmaniasis. Compared to the traditional homologous recombination gene targeting method, CRISPR-Cas9 has been shown to be...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32221923
DOI: 10.1007/978-1-0716-0294-2_14

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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